As of 2008 the largest commercial fleet of Douglas DC-3s belonged to Buffalo Airways in Canada- they have ten examples, but four are in storage at Yellowknife in the Northwest Territories. They are used for personnel and cargo transport throughout the northern part of Canada.
Four Star Air Cargo of San Juan, Puerto Rico, operates six DC-3s on freighter services throughout the Caribbean.
The largest passenger operator is currently Discovery Air Tours based at Sydney-Bankstown in Australia. They also operate six DC-3s on day and night scenic tour flights of the area.
Source: Air International, March 2009. "Douglas' Time Machine" by David Willis, p57.